community level physiological profiles biolog eco plates  (Biolog Inc)

Journal: Frontiers in Microbiology

Article Title: Unveiling the rhizosphere microbiome of Dendrobium : mechanisms, microbial interactions, and implications for sustainable agriculture

doi: 10.3389/fmicb.2025.1531900

Figure Lengend Snippet: Modern techniques for analyzing rhizosphere microbial communities.

Article Snippet: Various methods, including metagenomics, direct soil-phospholipid fatty acid (PLFA), colony-forming unit (CFU)-PLFA, and community-level physiological profiling (CLPP) (BIOLOG), each provide unique insights into microbial community composition and activity, which are crucial for understanding their ecological roles ( ).

Techniques: Environmental Sampling, Next-Generation Sequencing, DNA Sequencing, Sequencing, Functional Assay, Gene Expression, Activity Assay, In Situ

Journal: Microbiology Spectrum

Article Title: Influenza A virus infection alters the resistance profile of gut microbiota to clinically relevant antibiotics

doi: 10.1128/spectrum.03635-22

Figure Lengend Snippet: Changes in the metabolic profile of different cecal microbiota samples extracted from IAV- and mock-infected mice. Nutrient usage profiles are determined using Biolog EcoPlates. ( A ) Evaluation of significant differences in nutrient usage between the samples under study analyzing the data by an initial random forest iterative approach followed by a Boruta statistical analysis comparing all the measurements from individual nutrients. The importance of each nutrient is represented in a whisker-box plot. Boxes contain 50% of all aleatory arrangements close to the mean of the importance for each nutrient. The whiskers present the remaining arrangements. Red boxes indicate those nutrients with a null effect. Yellow boxes indicate evidence of changes, while green boxes mark the highest statistically significant differences in the ability to metabolize the indicated nutrients. The blue boxes mark the lower, mean, and shadowmax values in the Boruta analysis which also sets the cutoff limit. Green boxes correspond to statistically differential values with a P ≤ 0.05. Each condition contains the analysis of fecal samples from three mice. ( B ) Differences between the mean of IAV- and the mock-infected ones for each substrate usage in Biolog analysis. Metabolization of each substrate in the Biolog is performed using individual cecal ( n = 3). Individual values are presented in Table S1 at https://figshare.com/articles/dataset/Supplementary_Table_1_biolog_Spectrum_xlsx/24428692 (Biolog). Results are represented in AWCD (average well-color development) where the color gradient reference represents arbitrary difference units. Positions close to red represent the largest drops in nutrient usage when comparing IAV- vs mock-infected mice and in green the largest increases. ( C ) Representation of the P -value corresponding to the analysis of the differences between the IAV- and mock-infected groups for each of the substrates. The analysis was performed using the non-parametric Wilcoxon-Mann-Whitney analysis test. Results compare samples collected on days 3, 5, and 7. M: mock; I: IAV infections.

Article Snippet: One approach to determining the metabolic microbial profiles of communities associated with environmental changes is the analysis of their community-level physiological profile (CLPP) using Biolog EcoPlates ( , ).

Techniques: Infection, Whisker Assay, MANN-WHITNEY